Document -
Attana Application Note: Crude samples analysis - Screening and characterization of hybridoma supernatants
The objective of this application note was primarily to screen mouse IgG antibodies from crude samples for dissociation rates of bound antigen, and to determine detailed kinetic rate constants between the antigen and an antibody selected in the screen. Among other things, a method to capture mouse IgG from hybridoma supernatants was developed which can be replicated for capture of human IgGs.
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Topics
- Medical research
Categories
- label free
- antibodies
- crude samples
- biosensor
